Guzman, 61, is charged with 17 criminal counts including drug trafficking, conspiring to murder rivals, money laundering, and weapons offenses. "This prosecution demonstrates that we will apply all available resources to dismantle the leadership of unsafe drug cartels, wherever they operate, and will not rest until we have done so'".
The trial is set to begin Monday with jury selection.
The trial, which one expert says will cost the United States taxpayer more than $50m (£38m), will hear several hundred witnesses testify. Another was sent home after she said she couldn't be impartial, saying, "I feel very bad about drugs".
The judge has ordered the jury to remain anonymous and partially sequestered.
One potential juror was excused after she indicated the case made her feel unsafe.
It's a bagel with cream cheese, lox and capers, he replied.
Alleged Mexican drug kingpin Joaquin "El Chapo" Guzman somehow always managed to get away.
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He is accused of having a hand in dozens of murders, of using his drug cartel to smuggle more than 200 tons of cocaine into the United States, even pulling off running the massive operation from behind bars.
Prosecutors say the US government is seeking a $14 billion forfeiture order as part of the case. Guzman pleads not guilty, but the government has presented so much evidence―more than 300,000 pages and at least 117,000 audio recordings―that the defense complains they haven't had enough time to review it all.
At least some of the several hundred witnesses expected to testify are in witness protection programs or are already in jail, housed in special wings to protect them from reprisals. The Sinaloa cartel that Guzman founded in 1989 is still hugely powerful.
Recaptured drug lord Joaquin "El Chapo" Guzman is escorted by soldiers at the hangar belonging to the office of the Attorney General in Mexico City, Mexico on January 8, 2016. In the USA, opioid addiction has spiralled into an epidemic. In 2016, an average of 174 Americans died every day from drug overdoses. He's been held in solitary confinement in a high-security federal cell in Manhattan since January 2017, when Mexico agreed to allow his extradition to the United States for trial. The only visitors he is allowed are his three lawyers and twin, seven-year-old daughters, from whom he is separated by a thick glass screen.
Judge Brian Cogan has taken the extraordinary step of holding the trial behind closed doors.
Mexican druglord "El Chapo" Guzman is on the run from police after making a daring escape from Mexico's most secure prison. Re-arrested by Mexican marines in February 2014, he escaped again 14 months later.